Journal: Scientific Reports

Article Title: Identification of the optic recess region as a morphogenetic entity in the zebrafish forebrain

doi: 10.1038/srep08738

Figure Lengend Snippet: (A–C): Single confocal plane of a 48 hpf embryonic forebrain stained with DAPI (gray), immunolabeled for ZO-1 (A and B) or acetylated α-tubulin (C), in ventral (A and left panel in C) and lateral (B and right panel in C) views. The telencephalon (Tel), the optic recess region (ORR), and the hypothalamus (Hyp) form three distinct cellular regions in the secondary prosencephalon. The ORR is bordered by the dense fiber bundles, the anterior commissure (ac) and post-optic commissure (poc). The asterisk (*) in (B) (lateral view) indicates the enlargement of the dorsal ventricular lumen corresponding to the anterior intraencephalic sulcus (AIS). (D): Surface rendering of the shape of ventricle (white, in opacity), reconstructed from ZO-1 immunolabeling, overlapped on the shape of the brain (white, in transparency), reconstructed from DAPI staining. V = ventral, D = dorsal, L = lateral and F = frontal views. The 3D representation of both ventricle and brain facilitates the visualization of the convoluted ventricular organization in the forebrain. (E–F): Single confocal plane of a 48 hpf embryonic forebrain labeled for the neurogenic markers ccna2 , elavl3 and HuC/D in ventral (E) and lateral (F) views. Bottom left drawings show the level of corresponding optical planes. ccna2 -positive proliferative cells are concentrated around the ventricular zones, while elavl3 -positive differentiating and HuC/D-positive differentiated cells are located at the periphery of the neural tube. Scale bars = 50 μm.

Article Snippet: Mouse anti-ZO-1 (clone ZO1-1A12, Invitrogen) was used at a dilution of 1/200 to label the ventricles .

Techniques: Staining, Immunolabeling, Labeling

Journal: Scientific Reports

Article Title: Identification of the optic recess region as a morphogenetic entity in the zebrafish forebrain

doi: 10.1038/srep08738

Figure Lengend Snippet: (A–B): Single confocal plane of a 30 hpf embryonic forebrain stained with DAPI (gray) and immunolabeled for ZO-1, in ventral (A) and lateral (B) views. (C): Surface rendering of the shape of ventricle (white, in opacity), reconstructed from ZO-1 immunolabeling, overlapped on the shape of the brain (white, in transparency), reconstructed from DAPI staining. V = ventral, D = dorsal, L = lateral and F = frontal views. At 30 hpf the organization of the ventricular system is less complex than at 48 hpf. The frontal view displays a characteristic keyhole shape of the ventricle, with a larger lateral expansion of the ventral part compared to the dorsal part. (D–E): Single confocal plane of a 30 hpf embryonic forebrain labeled with the neurogenic markers ccna2 , elavl3 and HuC/D, in ventral (D) and lateral (E) views. Bottom left drawings show the level of the corresponding optical plane. The ccna2 staining is located around the ventricular walls but in wider territories than at 48 hpf. The elavl3 staining is detected adjacent to the ccna2 staining, also in wider territories than at 48 hpf. Accordingly, much fewer HuC/D-positive cells are detected than at 48 hpf, and they are all distributed at the periphery of the neural tube. The arrowheads in D indicate Hu-expressing cells located at the boundaries of telencephalon/ORR and ORR/hypothalamus. Scale bars = 50 μm.

Article Snippet: Mouse anti-ZO-1 (clone ZO1-1A12, Invitrogen) was used at a dilution of 1/200 to label the ventricles .

Techniques: Staining, Immunolabeling, Labeling, Expressing

Journal: Scientific Reports

Article Title: Identification of the optic recess region as a morphogenetic entity in the zebrafish forebrain

doi: 10.1038/srep08738

Figure Lengend Snippet: (A–B): Single confocal plane of a 24 hpf embryonic forebrain stained with DAPI (gray) and immunolabeled for ZO-1, in ventral (A) and lateral (B) views. diencephalon (Die); hypothalamus (Hyp); optic recess region (ORR); telencephalon (Tel). (C): Surface rendering of the shape of ventricle (white, in opacity), reconstructed from ZO-1 immunolabeling, overlapped on the shape of the brain (white, in transparency), reconstructed from DAPI staining. V = ventral, D = dorsal, L = lateral and F = frontal views. At 24 hpf the organization of the ventricular system is considerably simpler than at 48 hpf. The telencephalic ventricle rostral to the optic recess is not yet expanded (in contrast to 30 hpf). (D–E): Single confocal plane of a 24 hpf embryonic forebrain labeled for the neurogenic markers ccna2 , elavl3 and HuC/D, in ventral (D) and lateral (E) views. Bottom left drawings show the level of corresponding optical plane. The ccna2 staining is widely distributed while elavl3 and especially HuC/D expressions are restricted to small peripheral territories. The arrow heads in D indicate Hu-expressing cells located at the boundaries of telencephalon/ORR and ORR/hypothalamus. Scale bars = 50 μm.

Article Snippet: Mouse anti-ZO-1 (clone ZO1-1A12, Invitrogen) was used at a dilution of 1/200 to label the ventricles .

Techniques: Staining, Immunolabeling, Labeling, Expressing